JPSBR_Vol_6_Issue

ABSTRACT: A rapid sensitive, accurate, precise and reproducible validated alternative RP-UPLC method was developed for determination of less UV-active drug Artesunate in bulk and pharmaceutical dosage form using evaporative light scattering detection technique. The chromatographic estimation was carried out on Agilent 1290 series UPLC system with XBridge BEH C18 Column (50 x 2.1 mm, 2.5µm particle size) by using mobile phase Water (5mM Ammonium Acetate) and Acetonitrile with a gradient flow method of runtime as short as 3 min. The flow rate was 0.6 ml/min, temperature of the column compartment was maintained at ambient and detection was made by using Evaporative Light Scattering detector. The developed method was validated according to ICH guidelines with respect to linearity, accuracy, precision, specificity and robustness. The developed method was linear in concentration range of 100-300 ppm and the linear regression obtained was 0.9993. The proposed method was statistically evaluated and can be applied for routine quality control analysis of Artesunate in bulk and pharmaceutical dosage form. KEYWORDS: Artesunate, RP-UPLC, ELSD, Analytical method development, artemisinin, Antimalarial.

ABSTRACT: Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in mast cell degradation induced by clonidine in Rats. The effect of oral administration of hydroalcoholic extract of Cassia occidentalis leaves on clonidine induced mast cell degradation has been studied and is compared with the effect of oral administration of Sodium cromoglycate as standard on rats. A clonidine resulted in mast cell degradation. Supplementation with hydroalcoholic extract of Cassia occidentalis leaves significantly increased the percentage protection against mast cell degradation. The results indicate that the leaf of Cassia occidentalis is endowed with protected in mast cell degradation. KEY WORDS: Cassia occidentalis, clonidine, sodium cromoglycate, mast cell degradation, analysis of variance

ABSTRACT: The current study was aimed to investigate the potential of solid self-nanoemulsifying drug delivery system (S-SNEDDS) composed of Capmul MCM C8 (oil), Cremophor RH40 (surfactant) and transcutol P (co-surfactant) in improving the dissolution and oral bioavailability of Nateglinide (NTG). Liquid self-nanoemulsifying drug delivery systems (L-SNEDDS) were developed by using rational blends of components with good solubilizing ability for NTG which were selected based on solubility studies, further ternary phase diagram was constructed to determine the self-emulsifying region. The prepared L-SNEDDS formulations were evaluated to determine the effect of composition on physicochemical parameters like rate of emulsification, clarity, phase separation, thermodynamic stability, cloud point temperature, globule size and zeta potential. In vitro drug release studies of optimized L-SNEDDS showed almost 96.76±1.4% within 45 min. The globule size analysis revealed the formation of nanoemulsion (130 ± 1.6nm) from the optimized L-SNEDDS formulation. Optimized L-SNEDDS was incorporated into tabletting excipients to make optimized self-nanoemulsified tablet formulation. A three factor, three-level Box–Behnken design was used for the optimization procedure, with the amounts of X1 (maltodextrin), X2 (Kollidon VA 64), and microcrystalline cellulose (X3) as the independent variables, while Flowability index (Y1), Friability (%) (Y2), Disintegration time (min) (Y3) and Cumulative % of NTG released after 45 min (%) (Y4) as responses. The optimization model predicted 99.48% % release with X1, X2 and X3 levels of 224, 100 and 111, respectively. A new formulation was prepared according to these levels. The observed responses were in close agreement with the predicted values of the optimized formulation. KEYWORDS: Nateglinide, BCS class II, Self nanoemulifying drug delivery systems, maltodextrin, Kollidon VA 64, Optimization

ABSTRACT: Two simple, rapid, precise and accurate UV-spectrophotometric methods have been developed for determination of Meclizine Hydrochloride (MEH) and Pyridoxine Hydrochloride (PYH) by Simultaneous Equation Method (SEM) and Q-Analysis Method in combined tablet dosage form. The simultaneous equation method is based on measurement of absorbances at 220 nm and 295 nm and Q-Analysis is based on measurement of absorbances at isobestic wavelength i.e. 257 nm and wavelength of maximum absorption of one of the two components i.e. 295 nm for simultaneous estimation of Meclizine Hydrochloride and Pyridoxine Hydrochloride. The proposed methods obeyed Beer’s law in the concentration range of 5-30 μg/ml for MEH and 10-60 μg/ml for PYH. The proposed methods were validated and can be applied successfully for routine quality control analysis of MEH and PYH in bulk and pharmaceutical formulation. Key words: Simultaneous Equation Method, Q-Analysis, λmax, Validation, Meclizine Hydrochloride, Pyridoxine Hydrochloride.

ABSTRACT: Indole represents one of the most important heterocyclic ring which provides privileged scaffolds in drug discovery. Indole derivatives and its pharmacological significance provides tremendous opportunities to discover novel drugs with different modes of action. There are also amazing numbers of indole containing drugs in the market as well as compounds in clinical evaluation. This review serves as a comprehensive overview of currently published indole containing central nervous system acting agents with the main objectives in comprehensive listings of indole containing central nervous system drugs on market or compounds in clinical evaluation and to focus on recent developments of indole derivatives which are currently evaluated in experimental studies and their central nervous system activities. KEYWORDS: Indole, anticonvulsant, antidepressant, antianxiety, sedative, hypnotic

ABSTRACT: Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in paw edema induced by Carrageenan in Rats. The effect of oral administration of hydroalcoholic extract of Cassia occidentalis seeds on carrageenan induced inflammation has been studied and is compared with the effect of oral administration of Diclofenac sodium as standard on rats. A carrageenan resulted in paw edema. Supplementation with hydroalcoholic extract of Cassia occidentalis seeds significantly decreased paw edema. The results indicate that the seed of Cassia occidentalis is endowed with protected in paw edema. KEY WORDS: Cassia occidentalis, carrageenan, diclofenac sodium, paw edema, analysis of variance

ABSTRACT: This review describes an short impression of the recent trends in core shell HPLC columns. Core-shell technology provides an well-designed solution to the problem of maximizing column performance without generating extreme column back-pressure. They consist of an impermeable inner core surrounded by a layer of fully-porous silica and thus are morphologically quite different from conventional fully-porous silica particles.

The sub-2 micron particle size advantages are available in many publications. The use of these columns only on ultra-High pressure liquid Chromatography (UHPLC) Instrumentation having pump capacity is more than 900 bar. However, such instruments may not be readily available to every analyst. Core-Shell / Solid core practical alternative has been introduced which propose better efficiencies with using conventional HPLC. KEY WORDS: Core-Shell, UHPLC, porous silica, column performance.

ABSTRACT: Citalopram hydrobromide is used as an antidepressant drug. It is manufactured by 1-(4'-fluoropheny1)-phthalane -5-carbonitrile ( 5- Cyanophthalane) is Alkylated with 3-N,N-dimethylaminopropylchloride in presence of strong base to get Citalopram hydrobromide. Trace level of Cyanide as residual cyanide may remain in the product. Because during manufacturing process of 5- Cyanophthalane reaction with Cyanide salt in cyanation step. The residual Cyanide content in Citalopram hydrobromide API is determined by colorimetric procedure. The method is based on principle that in In the colorimetric measurement the cyanide is converted to cyanogen chloride, CNCl, by reaction with chloramine-T at a pH less than 8 without hydrolyzing to the cyanate. After the reaction is complete, color is formed on the addition pyridine-barbituric acid reagent. This colored compound, which is a complex of Quinquevalent Cyanide ,and determined spectrophotometrically.Beer's law was obeyed in the concentration ranges 3.0 to 15 ppm .The sensitivity of the method surpasses that of the reported spectrophotometric methods. The method was successfully applied for the determination of Cyanide in Citalopram Hydrobromide API as residual Cyanide in Parts per Million Level. KEY WORDS: Spectrophotometry, Citalopram Hydrobromide, API,Validation, Cynide, Beer law.

ABSTRACT: Two simple, rapid, precise and accurate UV-spectrophotometric methods have been developed for quatitative estimation of Paracetamol (PCM) and Aceclofenac (ACF) by simultaneous equation method and stability study method in Pharmaceutical tablet dosage form. The simultaneous equation method is based on measurement of absorbance at 247 nm and 275 nm as two wavelengths selected for determination of Paracetamol and Aceclofenac. The method obeyed Beer's law in the concentration range of 30-80 ?g/ml for PCM and 10-60 ?g/ml for ACF. Paracetamol and Aceclofenac were subjected to forced or stress degradation under different conditions recommended by ICH. The proposed methods were validated and can be applied successfully for routine quality control analysis of Paracetamol and Aceclofenac in bulk drugs and pharmaceutical dosage form. KEYWORDS: Simultaneous Equation Method, ?max, Validation, Paracetamol (PCM), Aceclofenac (ACF), Stability Study, Stress Degradation.