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Volume 8
Issue 2, June 2018
Development and Validation of Stability Indicating HPLC Method
for Simultaneous Estimation of Rifaximin and
Metronidazole Hydrochloride Tablet Dosage
Panchal Hemanshi*, Thakkar Divya
Stability Indicating RP-HPLC Method Development and Validation
for Simultaneous Estimation of Amitriptyline
Hydrochloride and Pantoprazole Sodium in Capsules
Sumit Sarvaiya*, Hiral Panchal
New Intellectual Property Rights Policy of India- will become
law changer?
Patel Nishith K.
Short Communication On Lercanidipin Formulation for Improved
Bioavailability
Jatin Panara
ABSTRACT:
A simple, rapid, economical, precise and accurate Stability indicating RP-HPLC method for simultaneous
estimation of Metronidazole and Rifaximin in their combined dosage form has been developed.
A
reverse phase high performance liquid chromatographic method was developed for the simultaneous
estimation of Metronidazole and Rifaximin in Their Combined Dosage Form has been developed. The
separation was achieved by LC- 20 AT C18 (250mm x 4.6 mm x 2.6 µm) column and Buffer (Potassium
Phosphate, pH 5.0): Acetonitrile (60:40) as mobile phase, at a flow rate of 1 ml/min. Detection was carried
out at 233 nm. Retention time of Rifaximin and Metronidazole were found to be 3.713 min and 6.107 min
respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for
Metronidazole 10-30 μg/ml and for Rifaximin 5-15 μg/ml. Developed method was found to be accurate,
precise and rapid for simultaneous estimation of Metronidazole And Rifaximin In Their Combined Dosage
Form. The drug was subjected to stress condition of hydrolysis, oxidation, photolysis and Thermal
degradation, Considerable Degradation was found in Thermal degradation. The proposed method was
successfully applied for the simultaneous estimation of both the drugs in commercial combined dosage
form.
KEY WORDS: Metronidazole ,Rifaximin , Stability indicating RP-HPLC Method, Validation.
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ABSTRACT:
A reverse phase high performance liquid chromatographic method was developed for the simultaneous
estimation of Pantoprazole sodium and Amitriptyline HCl in Capsules has been developed. The separation
was achieved by LC- 20 AT, Cosmocil C18 (250mm x 4.6 mm x 2.6 µm) column and Buffer 0.05 M KH2PO4
(pH 4.0): Methanol (60:40v/v) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at
242 nm. Retention time of Pantoprazole sodium and Amitriptyline HCl were found to be 3.187 min, and
5.497 min respectively. The method has been validated for linearity, accuracy and precision. Linearity
observed for Pantoprazole sodium (20-60 μg/ml) and for Amitriptyline HCl (5-15 μg/ml). Developed
method was found to be accurate, precise and rapid for simultaneous estimation of Pantoprazole sodium
and Amitriptyline HCl in their combined dosage form. The drugs were subjected to stress condition of
Acid, Base, oxidation, photolysis and Thermal degradation, Considerable Degradation was found in acidic
media for Amitriptyline HCl and photo degradation for Pantoprazole sodium. The degradation of
amitriptyline HCl and Pantoprazole sodium in acid, base, Oxidation, thermal and photolytic conditions
were found to be 18.221 %, 12.017%, 10.592%, 15.004%, 11.083% and for pantoprazole sodium 11.341%,
10.100%, 13.890%, 12.206%, 17.366% respectively. The proposed method was successfully applied for
the simultaneous estimation of both the drugs in commercial combined dosage form.
KEY WORDS: Pantoprazole sodium, Amitriptyline HCl, Stability indicating RP-HPLC Method, Anxipan
capsules.
ABSTRACT:
The New Intellectual Property Rights policy was announced by the Government of India in year 2016. This
policy lays out the future roadmap for Intellectual Property Rights in India. It envisages to provide a
comprehensive framework for Intellectual Property Rights by reviewing existing laws related to
Intellectual property and updating or improving them wherever required. After few legal cases the
internation business market seek to have change in perticular act that may boost indian business further.
So question arises whether these policies will remain addressing exhising law or will it be law changer in
future.
KEY WORDS: IPR, policy, India.
ABSTRACT:
Lercanidipine Hydrochloride is very effective hypertension treatment remedy but due to its poor physico-
chemical properties, the therapy is compromised. In current Research work the attempt was made to
improve solubility and dissolution of the same by lipid formulation. The formulation was tested for
various preformulation and post formulation test along with in invivo test on rats. The results showed
there is definitely chance of improvement in bioavailability of Lercanidipine Hydrochloride in human also.
KEY WORDS: Lercanidipine Hydrochloride, SEDDS, SMEDDS, Lipid formulation.
