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Volume 8
Issue 1, April 2018
First order Derivative Spectrophotometric Method for Determination of Minoxidil and Finasteride in
Pharmaceutical Dosage Form
Aishwarya Roy*, Madhuri Hinge, Shuchi Desai
Stability Indicating RP-HPLC Method Development and Validation for the Simultaneous Estimation
of Mupirocin and Metronidazole in Pharmaceutical Dosage
Priti Arapda *, Vanita Marvaniya , Pragnesh Patani
Development and Validation of Stability Indicating HPLC Method for Simultaneous Estimation of
Clonazepam and Propranolol HCl in their Combined Marketed Dosage Form
Samant Kapadiya*, Neha Tiwari, Pragnesh Patani
Review on various Analytical Method for Quantitative Determination of Etophylline and Theophylline
in Bulk and in Different Dosage Forms
Arina Macwan*, Neha Tiwari, Pragnesh Patani
ABSTRACT:
A
simple,
reliable,
accurate
and
precise
first
order
derivative
method
has
been
developed
and
validated
for
simultaneous
determination
of
Minoxidil
and
Finasteride
in
Pharmaceutical
dosage
form.
In
methanol
and
water,
the
quantitative
determination
was
carried
out
using
first
order
spectra
of
Minoxidil
at
300
nm
(ZCP
of
FIN)
and
Finasteride
and
at
228.63nm
(ZCP
of
MIN),
respectively.
The
linearity
for
MIN
and
FIN
was
obtained
in
the
concentration
range
5-25
μg/ml
and
0.1-0.5
μg/ml with 0.9990 and 0.9971 correlation coefficient, respectively. Method was validated according to ICH guideline.
KEY WORDS: Minoxidil (MIN), Finasteride (FIN), First order derivative method
![[x]](JPSBR_Vol_8_Issue_1_htm_files/close.png "Close")
ABSTRACT:
A
simple,
rapid,
economical,
precise
and
accurate
Stability
indicating
RP-HPLC
method
for
simultaneous
estimation
of
Metronidazole
and
Mupirocin
in
their
combined
dosage
form
has
been
developed.
A
reverse
phase
high
performance
liquid
chromatographic
method
was
developed
for
the
simultaneous
estimation
of
Metronidazole
and
Mupirocin.
In
their
combined
dosage
form
has
been
developed.
The
separation
was
achieved
by
LC-
20
AT
C18
(250mm
x
4.6
mm
x
2.6
µm)
column
and
buffer
(pH
3.5):
Methanol
(70:30)
as
mobile
phase,
at
a
flow
rate
of
1
ml/min.
Detection
was
carried
out
at
230
nm.
Retention
time
of
Metronidazole
and
Mupirocin
were
found
to
be
4.227
and
5.413
min,
respectively.
The
method
has
been
validated
for
linearity,
accuracy
and
precision.
Linearity
observed
for
Metronidazole
5-15
μg/ml
and
for
Mupirocin
10-30
μg/ml.
Developed
method
was
found
to
be
accurate,
precise
and
rapid.
The
drug
was
subjected
to
stress
condition
of
hydrolysis,
oxidation,
photolysis
and
thermal
degradation,
considerable
degradation
was
found
in
alkaline
degradation.
The
proposed
method
was
successfully
applied
for
the
simultaneous
estimation
of
both
the
drugs
in
commercial
combined
dosage form.
KEY WORDS: Metronidazole, Mupirocin, Stability indicating RP-HPLC Method, Validation.
ABSTRACT:
A
simple,
rapid,
economical,
precise
and
accurate
HPLC
method
for
simultaneous
estimation
of
Propranolol
HCl
and
Clonazepam
in
Their
Combined
Dosage
Form
has
been
developed.
A
Stability
indicating
reverse
phase
high
performance
liquid
chromatographic
method
was
developed
for
the
simultaneous
estimation
of
Propranolol
HCl
and
Clonazepam
in
their
combined
dosage
form.
The
separation
was
achieved
by
LC-
20
AT
C18
(250mm
x
4.6
mm
x
2.6
µm)
column
and
Buffer
(Potassium
Phosphate,
pH
4.5):
Methanol
(85:15)
at
a
flow
rate
of
1
ml/min.
Detection
was
carried
out
at
240
nm.
Retention
time
4.080
min
and
5.343
for
Propranolol
HCl
and
Clonazepam
respectively.
The
method
has
been
validated
for
linearity,
accuracy
and
precision.
Linearity
observed
for
Propranolol
HCl
40-120
μg/ml
and
for
Clonazepam
1-3
μg/ml.
Developed
method
was
found
to
be
accurate,
precise
and
rapid
for
simultaneous
estimation
of
Propranolol
HCl
and
Clonazepam
in
their
combined
dosage
form.
The
proposed
method
was
successfully
applied
for
the
simultaneous
estimation
of
both
the
drugs
in
commercial
combined
dosage
form.
The
drug
was
subjected
to
stress
condition
of
hydrolysis,
oxidation,
photolysis
and
Thermal
degradation,
Considerable
Degradation
was
found
in
Thermal
degradation.
The
proposed
method
was
successfully
applied
for
the
simultaneous estimation of both the drugs in commercial Combined dosage form.
KEY WORDS: Propranolol HCl, Clonazepam, Simultaneous Estimation, HPLC Method, Validation
ABSTRACT:
Theophylline
is
used
to
treat
lung
disease
and
COPD
(Bronchitis,
emphysema).
Etophylline
is
derivative
of
Theophylline,
used
as
anti-asthmatic
agent.
It
is
adenosine
antagonist
that
relaxes
muscles
of
bronchi.
They
are
under
the
class
of
drug
Xanthine.
These
medicines
need
to
be
used
regularly
for
relief
of
asthmatic
symptoms.
Various
analytical
methods
are
reported
for
estimation
and
stability
of
Theophylline
and
Etophylline
in
bulk and in different Formulation. A comprehensive Literature review is prepared for Theophylline and Etophylline.
KEY WORDS: Theophylline, Etophylline, RP-HPLC, SpectroPhotometric.
