Volume 5, Issue 5,
Pages no. 410-474
ISSN 2277-3681
Available online on 01 September 2015
J Pharm Sci Bioscientific Res. 2015; 5(5):410-474.
Copyright © 2014 JPSBR Publications
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Volume 5, Issue 5, Additional suppliment Issue, on occasion of International conference on
‘CURRENT RESEARCH IN SCIENCE AND ROLE OF SCIENCE IN HEALTH AWARENESS’
Pages no. 474-514
ISSN 2277-3681
Available online on 15 October 2015
J Pharm Sci Bioscientific Res. 2015; 5(5):474-514.
ABSTRACT:
A simple, rapid, economical, precise and accurate RP-HPLC method for simultaneous estimation of Cilostazole and Imipramine has
been developed. A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of
Cilostazole and Imipramine.. The separation was achieved by LC- 20 AT C18 (25 cm × 0.46 cm) Hypersil BDS column and Buffer (pH 4.5)-
Methanol (20:80)as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at 222 nm. Retention time of Cilostazole and
Imipramine were found to be 5.383 min and 3.153 min, respectively. The method has been validated for linearity, accuracy and precision.
Linearity observed for Cilostazole 6-18 ?g/ml and for Imipramine 6-18 ?g/ml. The percentage recoveries obtained for Cilostazole and
Imipramine were found to be in range of 99.61 ± 0.50 and 99.78 ± 0.65 respectively. Developed method was found to be accurate,
precise and rapid for simultaneous estimation of Cilostazole and Imipramine.. The proposed method was successfully applied for the
simultaneous estimation of both the drugs in commercial Combined dosage form.
KEYWORDS : Cilostazole imipramine Simultaneous estimation by RP-HPLC Method, Validation.
A simple, rapid, economical, precise and accurate RP-HPLC method for simultaneous estimation of Cilostazole and Imipramine has
been developed. A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of
Cilostazole and Imipramine.. The separation was achieved by LC- 20 AT C18 (25 cm × 0.46 cm) Hypersil BDS column and Buffer (pH 4.5)-
Methanol (20:80)as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at 222 nm. Retention time of Cilostazole and
Imipramine were found to be 5.383 min and 3.153 min, respectively. The method has been validated for linearity, accuracy and precision.
Linearity observed for Cilostazole 6-18 ?g/ml and for Imipramine 6-18 ?g/ml. The percentage recoveries obtained for Cilostazole and
Imipramine were found to be in range of 99.61 ± 0.50 and 99.78 ± 0.65 respectively. Developed method was found to be accurate,
precise and rapid for simultaneous estimation of Cilostazole and Imipramine.. The proposed method was successfully applied for the
simultaneous estimation of both the drugs in commercial Combined dosage form.
KEYWORDS : Cilostazole imipramine Simultaneous estimation by RP-HPLC Method, Validation.
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ABSTRACT:
A reversed-phase liquid chromatographic method has been developed and validated for estimation of Ornidazole and Diloxnide furoate in Tablet
dosage form. Chromatography was carried on C18 (25 x 0.46)cm ; 5µm) analytical column using mobile phase Buffer(pH 6.0) : Methanol (70:30
v/v) at a flow rate of 01.0 ml/min. The detection was carried out at 277 nm. The retention time of Ornidazole and Diloxanide furoate are found to
be 3.400 & 5.747 min respectively. Correlation co-efficient for Ornidazole and Diloxanide furoate was found to be 0.999 & 0.998 over a
concentration range of 25-75 µg/ml and 37.5-112.5 µg/ml respectively. The proposed method was validated with respect to linearity, accuracy,
precision, selectivity, and robustness. Recovery was found in the range of 99.63 - 100.05% and 99.80 - 100.25% for Ornidazole and Diloxanide
Furoate respectively. Statistical Analysis proves that the developed methods were successfully applied for the analysis of pharmaceutical
formulations and can be used for routine analysis of drugs in Quality Control laboratories.
KEY-WORDS: Ornidazole,Diloxanide Furoate, RP-HPLC, Mobile phase, Validation
A reversed-phase liquid chromatographic method has been developed and validated for estimation of Ornidazole and Diloxnide furoate in Tablet
dosage form. Chromatography was carried on C18 (25 x 0.46)cm ; 5µm) analytical column using mobile phase Buffer(pH 6.0) : Methanol (70:30
v/v) at a flow rate of 01.0 ml/min. The detection was carried out at 277 nm. The retention time of Ornidazole and Diloxanide furoate are found to
be 3.400 & 5.747 min respectively. Correlation co-efficient for Ornidazole and Diloxanide furoate was found to be 0.999 & 0.998 over a
concentration range of 25-75 µg/ml and 37.5-112.5 µg/ml respectively. The proposed method was validated with respect to linearity, accuracy,
precision, selectivity, and robustness. Recovery was found in the range of 99.63 - 100.05% and 99.80 - 100.25% for Ornidazole and Diloxanide
Furoate respectively. Statistical Analysis proves that the developed methods were successfully applied for the analysis of pharmaceutical
formulations and can be used for routine analysis of drugs in Quality Control laboratories.
KEY-WORDS: Ornidazole,Diloxanide Furoate, RP-HPLC, Mobile phase, Validation
ABSTRACT:
A reversed-phase liquid chromatographic method has been developed and validated for estimation of Paracetamol, Lornoxicam and
Serratiopeptidase in Tablet dosage form. Chromatography was carried on C18 (25cm x 0.46 cm) Hypersil BDS analytical column using
mobile phase Buffer (ammonium acetate pH5): Methanol (60:40) at a flow rate of 01.0 ml/min. The detection was carried out at 215 nm. The
retention time of Gresiofulvin is found to be 13min. Correlation co-efficient for PCM, LOR and SER was found to be 0.999, 0.998 and
0.998 respectively. Assay result of marketed formulation of PCM, LOR and SER was found to be in 98.67%, 98.51% and 98.668%
respectively. The proposed method was validated with respect to linearity, accuracy, precision, selectivity, and robustness. Recovery PCM,
LOR and SER was found in the range of 99.47% - 100.89%, 99.81% - 100.47%, 100.23% - 100.71% respectively. Statistical Analysis
proves that the developed methods were successfully applied for the analysis of pharmaceutical formulations and can be used for routine
analysis of drugs in Quality Control laboratories.
KEY-WORDS: Paracetamol, Lornoxicam, Serratiopeptidase, RP-HPLC, Mobile phase, Validation
A reversed-phase liquid chromatographic method has been developed and validated for estimation of Paracetamol, Lornoxicam and
Serratiopeptidase in Tablet dosage form. Chromatography was carried on C18 (25cm x 0.46 cm) Hypersil BDS analytical column using
mobile phase Buffer (ammonium acetate pH5): Methanol (60:40) at a flow rate of 01.0 ml/min. The detection was carried out at 215 nm. The
retention time of Gresiofulvin is found to be 13min. Correlation co-efficient for PCM, LOR and SER was found to be 0.999, 0.998 and
0.998 respectively. Assay result of marketed formulation of PCM, LOR and SER was found to be in 98.67%, 98.51% and 98.668%
respectively. The proposed method was validated with respect to linearity, accuracy, precision, selectivity, and robustness. Recovery PCM,
LOR and SER was found in the range of 99.47% - 100.89%, 99.81% - 100.47%, 100.23% - 100.71% respectively. Statistical Analysis
proves that the developed methods were successfully applied for the analysis of pharmaceutical formulations and can be used for routine
analysis of drugs in Quality Control laboratories.
KEY-WORDS: Paracetamol, Lornoxicam, Serratiopeptidase, RP-HPLC, Mobile phase, Validation
ABSTRACT:
Based on the literature review, it was found that a number of studies involving method development for estimation of GABAPENTIN and
NORTRIPTYLINE have been carried out in formulations/biological fluid. Thus, a number of analytical methods have been developed for
estimation of both drugs individually and in combination with other drugs. Review of literature reveals that no chromatographic and
Spectroscopic methods have been reported for simultaneous estimation of Gabapentin and Nortriptyline. in the Bulk drug and Pharmaceutical
dosage form. Therefore it is found that there is a need to develop an analytical method for simultaneous estimation of Gabapentin and
Nortriptyline. in formulations. So the present work is aimed for Development of simple and reproducible chromatographic (RP-HPLC) and
spectrophotometric (UV Spectroscopic method) method for simultaneous estimation of Gabapentin and Nortriptyline. Validation of the
developed chromatographic and spectroscopic method as per ICH guidelines.
KEY-WORDS: Gabapentin And Nortriptyline RP-HPLC, Mobile phase, UV Spectroscopt,Validation
Based on the literature review, it was found that a number of studies involving method development for estimation of GABAPENTIN and
NORTRIPTYLINE have been carried out in formulations/biological fluid. Thus, a number of analytical methods have been developed for
estimation of both drugs individually and in combination with other drugs. Review of literature reveals that no chromatographic and
Spectroscopic methods have been reported for simultaneous estimation of Gabapentin and Nortriptyline. in the Bulk drug and Pharmaceutical
dosage form. Therefore it is found that there is a need to develop an analytical method for simultaneous estimation of Gabapentin and
Nortriptyline. in formulations. So the present work is aimed for Development of simple and reproducible chromatographic (RP-HPLC) and
spectrophotometric (UV Spectroscopic method) method for simultaneous estimation of Gabapentin and Nortriptyline. Validation of the
developed chromatographic and spectroscopic method as per ICH guidelines.
KEY-WORDS: Gabapentin And Nortriptyline RP-HPLC, Mobile phase, UV Spectroscopt,Validation
ABSTRACT:
The present study was designated to evaluate the in vitro anthelmintic activity on Guru Nanak Chyawanprash. The gastrointestinal nematode,
Pheretima posthuma, has caused severe health problems to millions of people worldwide. Records show that many of the aqueous extracts
obtained from various parts of different herbal plants exhibited significant anthelmintic activity against Pheretima posthuma. This gave us a lead
to prepare new components resulting in the manufacturing of Guru Nanak Chyawanprash. The vermicidal activity in four respective
concentrations 25, 50, 75 and 100 mg/ml each were studied and in each concentration the time of paralysis and time of death of the worm were
determined. Guru Nanak Chyawanprash exhibited significant anthelmintic activity. At highest concentration of 100 mg/ml excellent anthelmintic
activity compared to Dabur Chyawanprash was shown. Guru Nanak Chyawanprash is very easy to prepare and is cost effective.
KEY WORDS: Guru Nanak Chyawanprash, Pheretima posthuma, Anthelmintic activity, Time of paralysis, Time of death.
The present study was designated to evaluate the in vitro anthelmintic activity on Guru Nanak Chyawanprash. The gastrointestinal nematode,
Pheretima posthuma, has caused severe health problems to millions of people worldwide. Records show that many of the aqueous extracts
obtained from various parts of different herbal plants exhibited significant anthelmintic activity against Pheretima posthuma. This gave us a lead
to prepare new components resulting in the manufacturing of Guru Nanak Chyawanprash. The vermicidal activity in four respective
concentrations 25, 50, 75 and 100 mg/ml each were studied and in each concentration the time of paralysis and time of death of the worm were
determined. Guru Nanak Chyawanprash exhibited significant anthelmintic activity. At highest concentration of 100 mg/ml excellent anthelmintic
activity compared to Dabur Chyawanprash was shown. Guru Nanak Chyawanprash is very easy to prepare and is cost effective.
KEY WORDS: Guru Nanak Chyawanprash, Pheretima posthuma, Anthelmintic activity, Time of paralysis, Time of death.
ABSTRACT:
The objective of this present study was to design bilayer tablet of two different drugs for separate release, evaluation of the same and
comparison dry granulation formulation with minor changes in components. Both layer of bilayer tablets comprised control release. In wet
granulation different type and amount of polymer were used for each layer. The formulated bilayer tablets were evaluated for pre compression
as well as post compression parameters including invitro_dissolution_study were carried out. The results showed that wet granulation of
formulated bilayer tablet carried out with different polymers viz. Gum acacia, Guar gum, Acrypol -971, HPMC_ K100M, eudragit_RSPO was
carried out and based on its release retarding properties. Based on drug release and release kinetics study final formulation was selected that
was further analysed for stability study. The accelerated stability study for 6 month showed affirmative results
KEY WORDS: Bilayer tablet formulation, fixed dose combination, drug release kinetics, stability study
The objective of this present study was to design bilayer tablet of two different drugs for separate release, evaluation of the same and
comparison dry granulation formulation with minor changes in components. Both layer of bilayer tablets comprised control release. In wet
granulation different type and amount of polymer were used for each layer. The formulated bilayer tablets were evaluated for pre compression
as well as post compression parameters including invitro_dissolution_study were carried out. The results showed that wet granulation of
formulated bilayer tablet carried out with different polymers viz. Gum acacia, Guar gum, Acrypol -971, HPMC_ K100M, eudragit_RSPO was
carried out and based on its release retarding properties. Based on drug release and release kinetics study final formulation was selected that
was further analysed for stability study. The accelerated stability study for 6 month showed affirmative results
KEY WORDS: Bilayer tablet formulation, fixed dose combination, drug release kinetics, stability study
ABSTRACT
Atorvastatin calcium is the drug used for treating hypercholesterolemia.Microemulsions are used as edge as potential drug deliveryvehicles
because of their thermodynamic stability, reversibility,simple manufacturing, and scale up feasibility, and do not requireany special equipment.
Microemulsionbased tablet of atorvastatin calcium was formulated using avicel 101 as career and aerosil as coating material. A32 full factorial
design was carried out to optimise oil: smix ratio and % SSG for the formulation of tablet. Regression analysis was carried out for dependent
variable and full and reduced polynomial equations ware generated for each variable. The response plot and counter plot were prepared for each
response to check effect of each variable on response. Using check point batch the polynomial equations were validated and optimised batch
were formulated using extensive grid search on overlay counter plot and polynomial equation. The optimised formulation was evaluated for flow
property, hardness, friability, disintegration time, emulsification time, and invitro drug release. Drug release kinetic of optimised batch showed
fickian diffusion type drug release. A stability study and comparison study with marketed formulation was carried out on optimise formulation.
KEYWORDS: Atorvastatin calcium, microemulsion, tablet dosage form, Liquid retention potential, Career: coat ratio
Atorvastatin calcium is the drug used for treating hypercholesterolemia.Microemulsions are used as edge as potential drug deliveryvehicles
because of their thermodynamic stability, reversibility,simple manufacturing, and scale up feasibility, and do not requireany special equipment.
Microemulsionbased tablet of atorvastatin calcium was formulated using avicel 101 as career and aerosil as coating material. A32 full factorial
design was carried out to optimise oil: smix ratio and % SSG for the formulation of tablet. Regression analysis was carried out for dependent
variable and full and reduced polynomial equations ware generated for each variable. The response plot and counter plot were prepared for each
response to check effect of each variable on response. Using check point batch the polynomial equations were validated and optimised batch
were formulated using extensive grid search on overlay counter plot and polynomial equation. The optimised formulation was evaluated for flow
property, hardness, friability, disintegration time, emulsification time, and invitro drug release. Drug release kinetic of optimised batch showed
fickian diffusion type drug release. A stability study and comparison study with marketed formulation was carried out on optimise formulation.
KEYWORDS: Atorvastatin calcium, microemulsion, tablet dosage form, Liquid retention potential, Career: coat ratio
ABSTRACT:
Reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Clonidine HCl and
Chlorthalidone. In Their Combined Dosage Form has been developed. The separation was achieved by LC- 20 AT C18 (250mm x 4.6
mm x 2.6 µm) column and Buffer (pH 4.0)-Methanol (70:30) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at
220 nm. Retention time of Clonidine HCl and Chlorthalidone were found to be 5.980 min and 4.150 min, respectively. The method has been
validated for linearity, accuracy and precision. Linearity observed for Clonidine HCl 1.5-4.5 ?g/ml and for Chlorthalidone 60-180 ?g/ml.
The percentage recoveries obtained for Clonidine HCl and Chlorthalidone were found to be in range of 99.56-101.02 and 99.11-
100.88 respectively. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Clonidine HCl In
Their Combined Dosage Form.
KEY-WORDS: Clonidine HCl, RP-HPLC, Mobile phase, Validation
Reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Clonidine HCl and
Chlorthalidone. In Their Combined Dosage Form has been developed. The separation was achieved by LC- 20 AT C18 (250mm x 4.6
mm x 2.6 µm) column and Buffer (pH 4.0)-Methanol (70:30) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at
220 nm. Retention time of Clonidine HCl and Chlorthalidone were found to be 5.980 min and 4.150 min, respectively. The method has been
validated for linearity, accuracy and precision. Linearity observed for Clonidine HCl 1.5-4.5 ?g/ml and for Chlorthalidone 60-180 ?g/ml.
The percentage recoveries obtained for Clonidine HCl and Chlorthalidone were found to be in range of 99.56-101.02 and 99.11-
100.88 respectively. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Clonidine HCl In
Their Combined Dosage Form.
KEY-WORDS: Clonidine HCl, RP-HPLC, Mobile phase, Validation
ABSTRACT:
Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in bronchospasm induced by histamine hydrochloride on Guinea pigs.
Exposure to an aerosol of 0.1% Histamine hydrochloride in closed chamber was given to induce bronchospasm on Guinea pigs. The effect of
oral administration of hydroalcoholic extract of Cassia occidentalis leaves on histamine induced bronchospasm has been studied and is
compared with the effect of oral administration of Ketotifen as standard on Guinea pigs. An aerosol of Histamine hydrochloride resulted in
bronchospasm as well as decreased pre-convulsion time. Supplementation with hydroalcoholic extract of Cassia occidentalis leaves
significantly increased the pre-convulsion time. The results indicate that the leaf of Cassia occidentalis is endowed with bronchospasmolytic
activity.
KEY WORDS: Cassia occidentalis, histamine, ketotifen, pre-convulsion time, analysis of variance
Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in bronchospasm induced by histamine hydrochloride on Guinea pigs.
Exposure to an aerosol of 0.1% Histamine hydrochloride in closed chamber was given to induce bronchospasm on Guinea pigs. The effect of
oral administration of hydroalcoholic extract of Cassia occidentalis leaves on histamine induced bronchospasm has been studied and is
compared with the effect of oral administration of Ketotifen as standard on Guinea pigs. An aerosol of Histamine hydrochloride resulted in
bronchospasm as well as decreased pre-convulsion time. Supplementation with hydroalcoholic extract of Cassia occidentalis leaves
significantly increased the pre-convulsion time. The results indicate that the leaf of Cassia occidentalis is endowed with bronchospasmolytic
activity.
KEY WORDS: Cassia occidentalis, histamine, ketotifen, pre-convulsion time, analysis of variance
ABSTRACT:
A novel combination of Montelukast sodium (MONT) and Acebrophylline (ACB) is used in the treatment of allergic rhinitis, bronchial asthma
and chronic obstructive pulmonary disease. A simple, accurate and reproducible HPTLC method has been developed and validated for the
simultaneous estimation of Montelukast sodium (MONT) and Acebrophylline (ACB) in their combined dosage form. Merck HPTLC aluminum
plates of silica gel G60 F254, (10 × 10 cm) was used for separation of combined drug with 250 ?m thickness using Chloroform : Ethyl acetate
: Methanol : Triethylamine (6 : 4.5 : 2.5 : 0.8, v/v/v/v) as mobile phase. HPTLC separation of the both drugs were carried out and followed by
densitometric measurement was performed in the absorbance mode at 272 nm. The drugs were resolved satisfactorily with Rf values of 0.23
± 0.01 and 0.83 ± 0.01 for ACB and MONT, respectively. The method was validated statistically as per ICH guidelines. The method showed
good reproducibility and recovery with % RSD less than 2. So, the proposed method was found to be simple, specific, precise, accuracy,
linear, and robust. Hence it can be applied for routine analysis of Montelukast sodium (MONT) and Acebrophylline (ACB) in pharmaceutical
formulations.
KEYWORDS: Montelukast; Acebrophylline; HPTLC; Simultaneous estimation
A novel combination of Montelukast sodium (MONT) and Acebrophylline (ACB) is used in the treatment of allergic rhinitis, bronchial asthma
and chronic obstructive pulmonary disease. A simple, accurate and reproducible HPTLC method has been developed and validated for the
simultaneous estimation of Montelukast sodium (MONT) and Acebrophylline (ACB) in their combined dosage form. Merck HPTLC aluminum
plates of silica gel G60 F254, (10 × 10 cm) was used for separation of combined drug with 250 ?m thickness using Chloroform : Ethyl acetate
: Methanol : Triethylamine (6 : 4.5 : 2.5 : 0.8, v/v/v/v) as mobile phase. HPTLC separation of the both drugs were carried out and followed by
densitometric measurement was performed in the absorbance mode at 272 nm. The drugs were resolved satisfactorily with Rf values of 0.23
± 0.01 and 0.83 ± 0.01 for ACB and MONT, respectively. The method was validated statistically as per ICH guidelines. The method showed
good reproducibility and recovery with % RSD less than 2. So, the proposed method was found to be simple, specific, precise, accuracy,
linear, and robust. Hence it can be applied for routine analysis of Montelukast sodium (MONT) and Acebrophylline (ACB) in pharmaceutical
formulations.
KEYWORDS: Montelukast; Acebrophylline; HPTLC; Simultaneous estimation
ABSTRACT:
Simple, precise and economical UV spectrophotometric methods have been developed for the estimation of Dapoxetine HCl in
pharmaceutical dosage form. Area under curve was integrated in the wavelength range of 285-305 nm. Calibration curves were
plotted. Beer’s law obeyed in the Concentration range 10-60μg/ml and with correlation coefficient of 0.9981. Accuracy and
precision studies were carried out and results were satisfactory. The proposed methods validated as per ICH analytical method
development guidelines. The results of the analysis were validated statistically.
KEY WORDS: Dapoxetine HCl, Beer’s law, Area under Curve, Validation, ICH guidelines
pharmaceutical dosage form. Area under curve was integrated in the wavelength range of 285-305 nm. Calibration curves were
plotted. Beer’s law obeyed in the Concentration range 10-60μg/ml and with correlation coefficient of 0.9981. Accuracy and
precision studies were carried out and results were satisfactory. The proposed methods validated as per ICH analytical method
development guidelines. The results of the analysis were validated statistically.
KEY WORDS: Dapoxetine HCl, Beer’s law, Area under Curve, Validation, ICH guidelines
ABSTRACT:
ABSTRACT: Water mediated green reaction of aromatic aldehyde with o-phenylenediamane in order to generate benzimidazoles
using boric acid as a catalyst in water at room temperature furnished benzimidazoles in good yields. This protocol corresponds
in short reaction time, low cost, high reaction yield
KEYWORDS: Aromatic aldehyde, o-phenylenediamane , Benzimidazoles ,Boric acid
using boric acid as a catalyst in water at room temperature furnished benzimidazoles in good yields. This protocol corresponds
in short reaction time, low cost, high reaction yield
KEYWORDS: Aromatic aldehyde, o-phenylenediamane , Benzimidazoles ,Boric acid
ABSTRACT:
Two simple, rapid, precise and accurate spectrophotometric methods have been developed for determination of Amlodipine
Besylate (AMB) and Hydrochlorothiazide (HCTZ) by simultaneous equation method and stability study method in combined
tablet dosage form. The simultaneous equation method is based on measurement of absorbance at 238 nm and 271 nm as
two wavelengths selected for quantification of Amlodipine Besylate and Hydrochlorothiazide. The method obeyed Beer’s law
in the concentration range of 5-30 μg/ml for Amlodipine and 2.5-15 μg/ml for Hydrochlorothiazide. Amlodipine and
Hydrochlorothiazide (Marketed Formulation) were subjected to stress degradation under different conditions recommended by
ICH. The proposed methods were validated and can be applied successfully for routine quality control analysis of AMB and
HCTZ in bulk and pharmaceutical formulation.
KEYWORDS: Simultaneous Equation Method, λmax, Validation, Amlodipine Besylate, Hydrochlorothiazide, Stability Study,
Stress Degradation.
Besylate (AMB) and Hydrochlorothiazide (HCTZ) by simultaneous equation method and stability study method in combined
tablet dosage form. The simultaneous equation method is based on measurement of absorbance at 238 nm and 271 nm as
two wavelengths selected for quantification of Amlodipine Besylate and Hydrochlorothiazide. The method obeyed Beer’s law
in the concentration range of 5-30 μg/ml for Amlodipine and 2.5-15 μg/ml for Hydrochlorothiazide. Amlodipine and
Hydrochlorothiazide (Marketed Formulation) were subjected to stress degradation under different conditions recommended by
ICH. The proposed methods were validated and can be applied successfully for routine quality control analysis of AMB and
HCTZ in bulk and pharmaceutical formulation.
KEYWORDS: Simultaneous Equation Method, λmax, Validation, Amlodipine Besylate, Hydrochlorothiazide, Stability Study,
Stress Degradation.
ABSTRACT:
The objective of present work is to formulate, optimize and evaluated of bilayer tablet of Lornoxicam and Thiocolchicoside. In this study
Lornoxicam is in immediate release layer and Thiocolchicoside is in sustain release layer. For immediate release layer sodium starch
glycolate was used as supredisintigration. And Sustain release layer Xanthan gum was used. The bilayer matrix tablet is evaluated by
measuring Hardness, Thickness, Diameter, in vitro drug release study, Weight variation, % drug content. FT-IR studies were also
indicating the absence of strong interactions between the components and suggesting drug-excipient compatibility in all the
formulations examined. 32 full factorial designs were used in present study for optimization. Amount of Xanthan gum and amount of
PVPK-30 M was used as an independent variable and % drug release was used as dependent variable. From the result formulation L4
was optimize which given the % drug release at 30 min 99.02 %. For sustain release layer check point batch was optimize which gives
% drug release at 8 hr 93.19 %.
KEY WORDS: Lornoxicam, Thiocolchicoside, Bilayer matrix, Rheumatoid arthritis.
Lornoxicam is in immediate release layer and Thiocolchicoside is in sustain release layer. For immediate release layer sodium starch
glycolate was used as supredisintigration. And Sustain release layer Xanthan gum was used. The bilayer matrix tablet is evaluated by
measuring Hardness, Thickness, Diameter, in vitro drug release study, Weight variation, % drug content. FT-IR studies were also
indicating the absence of strong interactions between the components and suggesting drug-excipient compatibility in all the
formulations examined. 32 full factorial designs were used in present study for optimization. Amount of Xanthan gum and amount of
PVPK-30 M was used as an independent variable and % drug release was used as dependent variable. From the result formulation L4
was optimize which given the % drug release at 30 min 99.02 %. For sustain release layer check point batch was optimize which gives
% drug release at 8 hr 93.19 %.
KEY WORDS: Lornoxicam, Thiocolchicoside, Bilayer matrix, Rheumatoid arthritis.
ABSTRACT:
Sickle cell anemia is an autosomal recessive type of hemoglobin disorder with which every year 300000 children are born. In sickle cell
anemia reduced life of red blood corpuscular the devastating and life threatening clinical sign and symptoms can occur in patients.
Therefore the management is major health concern. The main intend or current challenge has always been to improve the clinical
aspects of quality of life of the patients with low toxicity and safer and easy to administer therapy. T-AYU-HMTM is a novel timely tested
anti-sickling Traditional Ayurvedic Medicine designed on such a purpose to reduce clinical complications and enhance the quality of life.
The present study is single dose oral toxicity study of T-AYU-HMTM was performed to assess its Safety profile at high dose. Various
parameters like Body weight, mortality, and clinical signs of hematological parameters were assessed. Results show that T-AYU-HMTM
is safe up to the dose level of 2000mg/kg body weight.
KEY WORDS: Sickle cell anemia, Anti-sickling, oral toxicity study, traditional medicine, T-AYU-HMTM
anemia reduced life of red blood corpuscular the devastating and life threatening clinical sign and symptoms can occur in patients.
Therefore the management is major health concern. The main intend or current challenge has always been to improve the clinical
aspects of quality of life of the patients with low toxicity and safer and easy to administer therapy. T-AYU-HMTM is a novel timely tested
anti-sickling Traditional Ayurvedic Medicine designed on such a purpose to reduce clinical complications and enhance the quality of life.
The present study is single dose oral toxicity study of T-AYU-HMTM was performed to assess its Safety profile at high dose. Various
parameters like Body weight, mortality, and clinical signs of hematological parameters were assessed. Results show that T-AYU-HMTM
is safe up to the dose level of 2000mg/kg body weight.
KEY WORDS: Sickle cell anemia, Anti-sickling, oral toxicity study, traditional medicine, T-AYU-HMTM
ABSTRACT:
Saliva is a useful diagnostic fluid for periodontal diseases. Observing salivary biomarkers for oral and systemic diseases could become
an important complement to clinical examinations in preventing periodontal disease in early stage. Periodontal diseases are mainly the
inflammation of tooth supporting structures which affects gingiva, periodontal ligament, and alveolar bone. Recent findings indicate that
it is possible to detect biomarkers for oral diseases within saliva samples. The aim of this research will be to investigate if known
salivary biomarkers could be used for detection of periodontal diseases. Materials and Methods: A randomly selected sample of adults
(20–89 years), living in Thunder Bay, will be invited to participate. 100 individuals will be examined clinically using standard
examination procedures. Participants will be divided in 2 groups. First group will receive the results of the examination and if any
participant will be susceptible for periodontal disease, they will be advised to take professional care according to their results. Second
group will be the control group. Stimulated saliva samples will be collected and analysed for concentrations of IL- 1b, -6, -8, lysozyme,
matrix metalloproteinases (MMP)-8 and tissue inhibitor of metalloproteinase (TIMP)-1 using ELISA, immunofluorometric assay or
Luminex assays. The effect of using saliva as a biomarker will be evaluated by measuring clinical attachment loss (CAL) at the
beginning of the study and after 5 years. The measurements of CAL will be taken every 6 months during the period of 5 years. The
comparison of control group and the test groups will be done by analysis of variance (ANOVA) statistical test.
KEY WORDS: Saliva, Biomarker, Periodontal Disease, ANOVA, Luminex assays
an important complement to clinical examinations in preventing periodontal disease in early stage. Periodontal diseases are mainly the
inflammation of tooth supporting structures which affects gingiva, periodontal ligament, and alveolar bone. Recent findings indicate that
it is possible to detect biomarkers for oral diseases within saliva samples. The aim of this research will be to investigate if known
salivary biomarkers could be used for detection of periodontal diseases. Materials and Methods: A randomly selected sample of adults
(20–89 years), living in Thunder Bay, will be invited to participate. 100 individuals will be examined clinically using standard
examination procedures. Participants will be divided in 2 groups. First group will receive the results of the examination and if any
participant will be susceptible for periodontal disease, they will be advised to take professional care according to their results. Second
group will be the control group. Stimulated saliva samples will be collected and analysed for concentrations of IL- 1b, -6, -8, lysozyme,
matrix metalloproteinases (MMP)-8 and tissue inhibitor of metalloproteinase (TIMP)-1 using ELISA, immunofluorometric assay or
Luminex assays. The effect of using saliva as a biomarker will be evaluated by measuring clinical attachment loss (CAL) at the
beginning of the study and after 5 years. The measurements of CAL will be taken every 6 months during the period of 5 years. The
comparison of control group and the test groups will be done by analysis of variance (ANOVA) statistical test.
KEY WORDS: Saliva, Biomarker, Periodontal Disease, ANOVA, Luminex assays
ABSTRACT:
A series of 1-methyl imidazole carboxamide coupled with N-protected amino acid derivatives were synthesized, characterized and their
antimicrobial as well as antifungal properties were evaluated. These compounds were synthesized by coupling reaction in presence of
POCl3 and Pyridine and characterized using IR, 1H and mass spectroscopy. The synthesized compounds were screened for their in vitro
antimicrobial activity against S. aureus, E. coli, P. aeruginosa, S. typhimurium, F. oxysporum and A. alternata. Some of these compounds
exhibited moderate to good activity, where as some were found inactive, in comparison to pathogens being evaluated.
KEYWORDS: Imidazole, Amino acid, Carboxamide, Antibecterial, Antifungal & 5-Amino-1-methyl-1H-imidazole-4-carboxylic acid amide
A series of 1-methyl imidazole carboxamide coupled with N-protected amino acid derivatives were synthesized, characterized and their
antimicrobial as well as antifungal properties were evaluated. These compounds were synthesized by coupling reaction in presence of
POCl3 and Pyridine and characterized using IR, 1H and mass spectroscopy. The synthesized compounds were screened for their in vitro
antimicrobial activity against S. aureus, E. coli, P. aeruginosa, S. typhimurium, F. oxysporum and A. alternata. Some of these compounds
exhibited moderate to good activity, where as some were found inactive, in comparison to pathogens being evaluated.
KEYWORDS: Imidazole, Amino acid, Carboxamide, Antibecterial, Antifungal & 5-Amino-1-methyl-1H-imidazole-4-carboxylic acid amide
ABSTRACT:
Most of poorly water soluble fails to solubilize because of their particle size, to overcome this particle size reduction upto nano level
is most appropriate solution. Many times microsize have also its limitation therefore nano level size reduction is selected. Conversion
to nano level involves many physical as well as chemical methods, but in physical method drug may be degraded hence chemical
method is selected to achieve nanoparticles. Quasi emulsion solvent method is used to prepare nanoparticles of simvastatin by
using Pluronic F-68 stabilizer. And suitable concentrations were selected by using 32 factorial designs.
KEY WORDS: Nanoparticles, Quasi emulsion solvent technique.
is most appropriate solution. Many times microsize have also its limitation therefore nano level size reduction is selected. Conversion
to nano level involves many physical as well as chemical methods, but in physical method drug may be degraded hence chemical
method is selected to achieve nanoparticles. Quasi emulsion solvent method is used to prepare nanoparticles of simvastatin by
using Pluronic F-68 stabilizer. And suitable concentrations were selected by using 32 factorial designs.
KEY WORDS: Nanoparticles, Quasi emulsion solvent technique.
