Evaluation and Determination of Antifungal Potentials of Sap of Borassus Flabellifer
Tribhuvan Singh*, Akhilesh Kumar Verma, Syed Imran Ul Haq, N. Mounika
Quantification of Lupeol and Betulin in Ougenia Dalbergioides Bark by Column Chromatography and TLC
Mariyan R. Patel*, Namrata Rajput, Hiteksha S. Panchal, Himani B. Dalwadi
Volume 7, Issue 1
Pages no. 111-177
Available online on 01 Jan 2017
ISSN 2277-3681
J Pharm Sci Bioscientific Res. 2017; 7(1):111-177.
Formulation and In-vitro Evaluation of Valsartan Fast Dissolving Tablets by Sublimation Technique
Basawaraj S. Patil
Synthesis of Benzoxazole Derivatives at Room Temperature using PEG-SO3H as the Heterogeneous Catalyst
Yogesh M. Patel, *, Rajendra R. Patel, Umesh P. Tarpada, Vivek N. Dave
Formulation and Evaluation of Natural Lipsticks Prepared from Bixa Orellana Seeds and Daucus Carota Root Extract
and their Comparative Study
Verma Shashi*, Tripathi Devika, Tiwari Ritesh Kumar
Development and Validation of Ultra Violet Spectrophotometric Assay Method of Spironolactone by Hydrazine
Derivatization
Hozyfa A. Hamdan, Ahmed E. Mohammed
Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of Nebivolol
Hydrochloride and Cilnidipine in Tablet Dosage Form
Nisha Patel, R. S. Mehta, A. D. Caption, V. V. Karkhanis, P. D. Patel, A. A. Chavda
Copyright © 2014 JPSBR Publications
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Studies on Transition Metal Chelates of Azo Ligand Containing Bon Acid
Bhavana K.Patel, Sanjay D.Patel
Nanoemulsion: A Novel Approach in Various Pharmaceutical Applications
Jyotsana Suyal
Stability Indicating HPLC Method for Simultaneous Estimation of Pregabalin and Methylcobalamin from Combined
Dosage Form
Ankita J. Patel*, Hiren Kadikar
ABSTRACT:
The present study was conducted to evaluate in vitro Antifungal activity of Sap of Borassus Flabellifer . The Fungal strain like
C. Albicans and A. Niger cause wide range of health complications to most people worldwide. Herbal products,
phytomedicines, herbal remedies have been showing a remarkable growth due to less toxicity and side effects compared to
synthetic medicines. We conducted research to determine Antifungal activity of Sap of Borassus Flabellifer by evaluating in
four different volumes (0.25, 0.50, 0.75, 1.0ml) of Sap and zone of inhibition. We found that the sap of Borassus Flabellifer
exhibits significant Antifungal activity of 14 to 26 mm zone of inhibition after 36hrs. By this research we found the Sap of
Borassus Flabellifer exhibited significant Antifungal activity.
KEY WORDS: Borassus Flabellifer ; Antifungal Activity; Zone of Inhibition.
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ABSTRACT:
Ougenia dalbergioides Benth. (fabaceae) commonly known as sandan. It is reported to have good medicinal values in traditional system of
medicine. According to Ayurveda the bark is used in dysentery, leucoderma, urinary discharge, ulcers, skin disease and blood disease,
biliousness, and anaemia. A systematic HPTLC fingerprinting on the bark of Ougeinia dalbergioides has not been reported in literature to our
knowledge. So, simultaneous HPTLC quantification of the bark of Ougeinia dalbergioides was carried out. Two compounds were isolated from
the methanolic extract of Ougeinia dalbergioides by preparative TLC using toluene: chloroform: methanol (8.8:8.8:2.2) as mobile phase. These
compounds were found to be triterpene by spraying with anisalehyde-H2SO4 as spraying reagent. Melting point, Co-TLC with standards and
Spectroscopic data of UV and FTIR confirmed triterpene as betulin and lupeol. The simultaneous HPTLC quantification of lupeol and betulin in
acetone and methanol extract were shows that amount of lupeol in acetone extract and methanol extract were 9.9 µg /mg of extract and 8.3 µg
/mg of extract respectively. The method is validated for the parameters like linearity, accuracy, precision, specificity, limit of detection and limit of
quantification. From the data it was found that the method was linear, accurate, précised and specific.
KEYWORDS: Ougenia dalbergioides, column chromatography, UV, IR, HPTLC, lupeol, betulin
ABSTRACT:
Valsartan is an angiotensin II receptor antagonist and is widely used in the management of hypertension to reduce
cardiovascular mortality in patients with left ventricular dysfunction following myocardial infarction, and in the management of
heart failure. The purpose of this investigation was to develop fast dissolving tablets (FDTs) of Valsartan by sublimation
technique using camphor as subliming agent together with crospovidone (CP) as superdisintegrant. The prepared formulations
were evaluated for pre-compressional and post-compressional parameters. The compatibility of drug with other ingredients was
checked by FTIR studies, the results revealed that there was no interaction between dug and other excipients. Effect of
subliming agent camphor and superdisintegrant crospovidone on disintegration time, wetting time, Water absorption ratio, drug
content in-vitro release and stability parameters have been studied. Disintegration time and dissolution parameters (t50% and
t90%) decreased with increase in the level of camphor and crospovidone. Stability study carried out as per ICH guidelines for
three months and results revealed that upon storage disintegration time of tablets decreased significantly (p<0.05). It is
concluded that fast dissolving tablets of Valsartan could be prepared by sublimation technique.
KEY WORDS: Valsartan, fast dissolving tablet, crospovidone, camphor, super disintegrant.
ABSTRACT:
PEG-SO3H was found to be an effective heterogeneous catalyst for the one pot synthesis of various benzoxazole derivatives from
condensation reaction between o-aminophenol with various aromatic aldehydes in ethanol to afford excellent yields. Synthesis was
attempted at room temperature using ethanol as the solvent. Heterogeneity of the catalyst allowed its recycling for five times with
almost retention in catalytic activity. Reaction carried out at room temperature shows special advantageous because it has
contribution in the green chemistry aspect.
KEYWORDS: Benzoxazole; heterogeneous catalyst; o-aminophenol, aromatic aldehyde.
ABSTRACT:
Cosmetics are incredible in demand since historical time till day .Lipstick formulations are most widely used to enhance the
beauty of lips and add glamour to touch to the makeup. With this aim and objectives, an attempt was made to formulate natural
lipsticks by using coloring pigments of Bixa orellana linn seeds and Daucus carota root and the lipsticks were evaluated for their
organoleptic properties such as spreading, hardness, shine and gloss and found to be satisfactory product to give attractive
beauty .The preparation of this lipsticks with the natural ingredients like Bixa seeds, Carrot root, Olive oil, Ripe fruit powder of
shikakai. Due to various adverse effects of available synthetic preparation ,the present work was conceived by us to formulate a
herbal lipsticks having minimal or no side effects which will extensively used by the women of our communities with great surety
and satisfaction.
KEYWORDS: Bixa orellana, Daucus carota, Herbal lipsticks, Formulation, Cosmetics.
ABSTRACT:
A simple, precise and accurate UV spectrophotometric method has been developed for determination of spironolactone in raw
material and tablet dosage form. Ethanol was used as solvent for the method. The wavelength 251.5 nm was chosen for
determination of spironolactone. Beer's low was obeyed in concentration range 20-30 ppm. The assay percentage of spironolactone
in tablet dosage form was found to be (97.15±0.282) %. The % of recovery was found to be (100.20-102.65) %. The limit of detection
(LOD) and limit of quantization (LOQ) were found to be 0.028 ppm and 0.0858 ppm respectively. The results of analysis have been
validated statistically and recovery studies confirmed the accuracy of accuracy of developed method which was carried out
according to ICH guidelines.
KEY WORDS: Spironolactone, UV spectrophotometric, Method development and Validation.
ABSTRACT:
A simple, specific, accurate and stability-indicating reversed phase high performance liquid chromatographic method was
developed for the simultaneous determination of Nebivolol hydrochloride and Cilnidipine in tablet dosage form. A Spheri-5-RP-18
column having 250×4.6 mm i.d., with mobile phase composed of Methanol: 20 mM Ammonium acetate (85:15, v/v; pH 4.0 adjusted
with Formic acid. The retention times of Nebivolol hydrochloride and Cilnidipine were found to be 3.4 min and 8.4 min, respectively.
Linearity was established for Nebivolol hydrochloride and Cilnidipine in the range of 5-30 ?g/ml and 10-60 ?g/ml respectively. The
percentage recoveries for Nebivolol hydrochloride and Cilnidipine were found to be in the range of 99.22-99.91% and 99.81-
100.82% respectively. The limit of detection for Nebivolol hydrochloride and Cilnidipine were found to be 0.80 ?g/ml and 1.73 ?g/ml
respectively. The limit of quantitation for Nebivolol hydrochloride and Cilnidipine were found to be 2.43 ?g/ml and 5.26 ?g/ml
respectively. Both the drugs were subjected to acid, alkali, oxidation, dry heat and photolytic degradation. The degradation studies
indicated, Nebivolol hydrochloride to be susceptible to oxidation while Cilnidipine showed degradation in oxidative and photolytic
condition. The degraded products peaks were well resolved from the pure drug peak with significant differences in their retention
time values. The proposed method was validated and successfully applied to the simultaneous estimation of Nebivolol
hydrochloride and Cilnidipine in bulk drugs and formulations.
KEY WORDS: Nebivolol hydrochloride, Cilnidpine, degradation, reversed phase high performance liquid chromatography, stability
indicating method, Validation
ABSTRACT:
New azo ligand i.e. 3-hydroxy-4-((5-(furan-2-yl)-1,3,4-oxadiazol-2-yl)-2-naphthoic acid (DAFOBA) was prepared by coupling reaction
between the diazonium salt of 2-amino-5-(furan-2-yl)-1,3,4-oxadiazol (AFO) and bon acid (BA). The structure of azo ligand was
characterized on the basis of elemental analyses, NMR, FT-IR and UV-Vis spectroscopic techniques. Transition metal chelates of
prepared azo ligand have been synthesized and characterized. The structure of metal chelates has been confirmed by using elemental
Analysis along with metal content determination, FT-IR, reflectance spectra as well as magnetic susceptibility measurements.
Analytical data revealed that all the complexes exhibited 1:2 metal-ligand ratios. Also the biological evaluation of all the synthesized
compounds was carried out.
KEY WORDS: 2-amino-5-(furan-2-yl)-1,3,4-oxadiazol, Bon acid, Dyeing study, spectral study, Biological activities.
ABSTRACT:
Nanoemulsions are nano sized emulsions that are used under high investigation as drug carriers for improving the delivery of therapeutic
agents. This advanced technology is used for the systemic delivery of biologically active agents for controlled delivery and targeting. These
are the novel drug delivery system consisting of emulsified oil and water system which mean droplet diameter ranging from 5 to 200 nm
.These emulsions are easily produced in large quantities by mixing a water immiscible oil phase into an aqueous phase with high stress.
These are prepared from surfactants, cosurfactants or cosolvents. In this review various aspects of nanoemulsion are highlighted i.e.
advantages, disadvantages, method of preparation, characterization techniques like polydispersity, particle size, zeta potential, drug
content with special emphasis on various application of nanoemulsion in different areas such as in cancer treatment, drug targeting, as a
vehicle for transdermal drug delivery, self nano emulsifying drug delivery system
KEY WORDS: Nanoemulsion, Cosurfactant, Cosolvent, emulsified oil.
ABSTRACT:
A stability indicating HPLC method has been described for the estimation of Pregabalin and Methylcobalamin in combined Dosage form using Agilent
technologies 1290 series HPLC and Column Merck Purosphere encapped C18 column (250 X 4.6mm,5?m), in isocratic mode consisting of DAD
detector with mobile phase Water :Methanol of( 65: 35)%v/v at flow rate of 1ml/min. The effluent is monitored at 205 nm. The peaks obtained were
sharp having clear baseline with retention time 5 mins and 11 mins for Pregabalin and Methylcobalamin. The linearity observed in the range of
1125µg/ml to 4875µg/ml and the correlation co- efficient value of drug was found to be 0.999, which shows the good linearity between concentration
of drug and response. The % C.V of precisions was less than 2, which indicate that the method has good degree of reproducibility. Degradation study
performed using Acid, alkali & water hydrolysis, Oxidation, thermal, Photo and UV degradation in which degradation product separated in case of
alkali, hydrolysis, Acid, Alkali, Photo and UV degradation and while in Humidity degradation no relative degradation was observed. Pregabalin and
Methylcobalamin peaks is spectrally pure in each case and peak purity is obtained more than 990. For routine analysis, formulations containing
Pregabalin and Methylcobalamin LOD, LOQ were found to be 12.11 µg /ml and 0.26 µg /ml, 36.69 µg /ml and 0.81 µg /ml respectively. Thus the
proposed method is suitable.
KEY WORDS: Pregabalin, Methylcobalamin, HPLC Method, Degradation.
