ABSTRACT: Two simple, accurate, and precise spectrophotometric method have been developed for Amitriptyline hydrochloride (AMI) and Perphenazine (PER) in their mixture. Amitriptyline hydrochloride has absorbance maxima at 239 nm and Perphenazine absorbance maxima at 257 nm. Method-A area under curve (AUC) method, which involves the calculation of integrated value of the Absorbance with respect to wavelength between 230-242nm for Amitriptyline hydrochloride and 249-259nm for Perphenazine. Method-B First order derivative spectrophotometric method involves the derivatisation of the Zero absorption spectra for the First absorption spectra. The Zero crossing point of Amitriptyline hydrochloride and Perphenazine at 254.9nm and 236.6nm was obtained respectively. Beer’s law is obeyed in the concentration range of 5-25 µg/ml and 2-10 µg/ml for Amitriptyline hydrochloride and Perphenazine for two methods. The accuracy and precision of the methods were determined and validated statistically. All the methods sowed good reproducibility and recovery with % RSD less than 1. All method was found to be rapid, precise and accurate and can be successfully applied for the routine analysis of Amitriptyline hydrochloride and Perphenazine in their mixture. KEY WORDS: Amitriptyline hydrochloride, Perphenazine, Area Under curve method, First order derivative spectroscopic method.

ABSTRACT: The   current   short   communication   provides   brief   history   of   Patent   cooperation   treaty   along   with   that   it   provides   that   it   is   an international   treaty   that   allows   patent   protection   for   its   all   member   countries   rather   than   individual   filing   in   individual   countries.   It   is   a filing   system   rather   than   international   granting   system.   That   provides   time   for   filing,   patentability   assessment,   cost   saving   and avoids unnecessary time delay. It has harmonize the filing system up to some extent throughout the member countries.   KEY WORDS: International Patent filing, PCT, WIPO

ABSTRACT: A   UV   Spectrophotometric   method   was   developed   by   applying   Quality   by   Design   (QbD)   approach   for   the   determination   of   Chlorpheniramine Maleate   in   its   pharmaceutical   dosage   form.   In   this   research   work,   three   critical   method   variables   which   are   solvent,   scanning   speed   and   sampling interval   were   assessed   by   applying   Design   of   Experiment   (DoE)   approach   and   was   also   optimized.   Two   wavelengths   which   are   261   nm   and   245 nm   was   selected   using   water,   0.1   N   HCl   and   0.1   N   NaOH   as   solvents   (later   HCL   was   selected   as   final   solvent).   Linearity   was   observed   at concentration   of   10µg/ml   –   50µg/ml.   The   correlation   coefficients   for   Chlorpheniramine   maleate   at   both   wavelengths   is   0.9995   and   0.999.   The results of method validation were in the acceptable range as per ICH guidelines. KEY WORDS: Chlorpheniramine Maleate, UV spectrophotometry, Quality by design, Validation parameters, Pharmaceutical formulation.